Human Anti-HIV-1 gp41 Recombinant Antibody (clone 4E10)
CAT#: PABL-192
Recombinant Human Antibody (4E10) is capable of binding to HIV-1 gp41 transmembrane subunit, expressed in HEK 293 cells. Expressed as the combination of a heavy chain (HC) containing VH from anti-HIV-1 gp41 transmembrane subunit mAb and CH1-3 region of human IgG1 and a light chain (LC) encoding VL from anti-HIV-1 gp41 transmembrane subunit mAb and CL of human light chain. Exists as a disulfide linked dimer of the HC and LC hetero-dimer under non-reducing condition. This broadly neutralizing antibody could interact with membrane lipids, but the antibody regions contacting lipids and its orientation with respect to the viral membrane are unknown.
Published Data
Tested Data
Figure 1 Neutralizing activity of MPER-specific IgA1 on primary and laboratory HIV-1 strains. 4E10 and 2F5 were used as positive controls. Dose-dependent neutralization was performed within a concentration range of 0.156–10 mg/ml.
Benjelloun, F., Oruc, Z., Thielens, N., Verrier, B., Champier, G., Vincent, N.,... & Genin, C. (2016). First Membrane Proximal External Region–Specific Anti-HIV1 Broadly Neutralizing Monoclonal IgA1 Presenting Short CDRH3 and Low Somatic Mutations. The Journal of Immunology, 1600309.
Figure 2 Binding of MPER-specific IgA on SupT1/LAI-infected cells. Number of IgA+/p24+ SupT1 cells was analyzed by flow cytometry. 4E10 was used as positive control and labeling with anti-p24+ + anti-IgA-PE as negative control. The ratio of the mean fluorescence of IgA+/p24+ SupT1 cells on the mean fluorescence of IgA2/p24+ SupT1 cells is indicated. Uninfected cells were used as negative control.
Benjelloun, F., Oruc, Z., Thielens, N., Verrier, B., Champier, G., Vincent, N.,... & Genin, C. (2016). First Membrane Proximal External Region–Specific Anti-HIV1 Broadly Neutralizing Monoclonal IgA1 Presenting Short CDRH3 and Low Somatic Mutations. The Journal of Immunology, 1600309.
Figure 3 Competition assay for C6.11 and F4.30 binding to gp120c41 in the presence of 2F5 or 4E10. gp120c41-coated wells were preincubated with 2F5 or 4E10 at different concentrations (0–20 mg/ml). C6.11 and F4.30 were used at 5 mg/ml. Assays were performed in triplicate.
Benjelloun, F., Oruc, Z., Thielens, N., Verrier, B., Champier, G., Vincent, N.,... & Genin, C. (2016). First Membrane Proximal External Region–Specific Anti-HIV1 Broadly Neutralizing Monoclonal IgA1 Presenting Short CDRH3 and Low Somatic Mutations. The Journal of Immunology, 1600309.
Figure 4 Kinetic analysis of the interaction between MPER-specific IgA and recombinant gp120c41. C6.11 (5–80 nM), F4.30 (0.5–16 nM), and 4E10 (0.5–8 nM) were injected over 8200 RU of immobilized gp120c41 in PBS containing 0.005% surfactant P20.
The binding signals shown were obtained by double referencing (subtraction of the reference surface and of buffer blank injection signals). Fits are shown as dotted lines and were obtained by global fitting of the data using a 1:1 Langmuir binding model.
Benjelloun, F., Oruc, Z., Thielens, N., Verrier, B., Champier, G., Vincent, N.,... & Genin, C. (2016). First Membrane Proximal External Region–Specific Anti-HIV1 Broadly Neutralizing Monoclonal IgA1 Presenting Short CDRH3 and Low Somatic Mutations. The Journal of Immunology, 1600309.
Figure 5 Screening of the neutralizing activity of MPER-specific IgA on COT6.15 pseudoviruses and COT6.15 mutants.
MPER sequence is shown in each panel. For each mAb and mutant, the value of SD is calculated for the neutralization from two independent experiments realized in triplicate. The critical residues for neutralization are indicated in red.
Benjelloun, F., Oruc, Z., Thielens, N., Verrier, B., Champier, G., Vincent, N.,... & Genin, C. (2016). First Membrane Proximal External Region–Specific Anti-HIV1 Broadly Neutralizing Monoclonal IgA1 Presenting Short CDRH3 and Low Somatic Mutations. The Journal of Immunology, 1600309.
Figure 6 Interactions of 10E8 with lipid bilayers.
A liposome flotation assay was used to detect binding to HIV-1-like membranes at 37°C by various antibodies, i.e., 17b, 4E10, 10E8, 10E8-F100aA, and 10E8-W100bA. Seven fractions throughout the sucrose gradient (from top [t] to bottom) after centrifugation were collected and analyzed by Western blotting. The heavy and light chains of antibodies are indicated by H and L, respectively.
Chen, J., Frey, G., Peng, H., Rits-Volloch, S., Garrity, J., Seaman, M. S., & Chen, B. (2014). Mechanism of HIV-1 neutralization by antibodies targeting a membrane-proximal region of gp41. Journal of virology, 88(2), 1249-1258.
❮
❯
ELISA
Figure 1 Human Anti-HIV-1 gp41 Recombinant Antibody (clone 4E10) (PABL-192) in ELISA
ELISA analysis of PABL-192 was performed by coating with Recombinant HIV1 gp41 protein.
The secondary antibody: HRP-Goat anti-Human IgG
WB
Figure 2 Human Anti-HIV-1 gp41 Recombinant Antibody (clone 4E10) (PABL-192) in WB
Western blot analysis of PABL-192 was performed by loading Recombinant HIV1 gp41 protein.
PABL-192 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat anti-Human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
DB
Figure 3 Human Anti-HIV-1 gp41 Recombinant Antibody (clone 4E10) (PABL-192) in Dot Blot
Dot Blot analysis of PABL-192 was performed by coating with Recombinant HIV1 gp41 protein.
TAB-422CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG (H+L)
❮
❯
Specifications
- Host Species
- Human
- Type
- Human IgG
- Species Reactivity
- HIV-1
- Clone
- 4E10
- Applications
- WB, Neut, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Neut, FC, ELISA and WB. For details, refer to published data.
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for PABL-192. Click the button above to contact us or submit your feedback about this product.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-192, RRID: AB_3111636)
Copy citation
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 4E10"
- CAT
- Product Name
See other products for "HIV-1 gp41"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-486-F(E) | Recombinant Anti-HIV-1 gp41 Antibody Fab Fragment | ELISA, FacS, FuncS | Fab |
| PFBL-185 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 8062); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| PFBL-186 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 2F5); Fab Fragment | WB, Neut, FuncS | Human Fab |
| PFBL-187 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 3BC176); Fab Fragment | Neut, FuncS | Human Fab |
| PFBL-188 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 3BC315); Fab Fragment | Neut, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-486-S(P) | Recombinant Human Anti-HIV-1 gp41 Antibody scFv Fragment | IF, Neut, FuncS | scFv |
| PSBL-185 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 8062); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| PSBL-186 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 2F5); scFv Fragment | WB, Neut, FuncS | Human scFv |
| PSBL-187 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 3BC176); scFv Fragment | Neut, FuncS | Human scFv |
| PSBL-188 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 3BC315); scFv Fragment | Neut, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-185 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 8062) | WB, ELISA, FuncS | Human IgG |
| PABL-186 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 2F5) | WB, Neut, FuncS | Human IgG |
| PABL-187 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 3BC176) | Neut, FuncS | Human IgG |
| PABL-188 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone 3BC315) | Neut, FuncS | Human IgG |
| PABL-189 | Human Anti-HIV-1 gp41 Recombinant Antibody (clone m66) | Neut, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-025LC-S(P) | Anti-HIV-1 gp41 Recombinant Antibody scFv Fragment (4E10-IgG3) | ELISA | Human antibody |
| MRO-026LC-S(P) | Anti-HIV-1 gp41 Recombinant Antibody scFv Fragment (4E10-IgG1) | ELISA | Human antibody |
| MRO-040LC-S(P) | Anti-HIV-1 gp41 Recombinant Antibody scFv Fragment (M66.6) | ELISA | Human antibody |
| MRO-025LC-F(E) | Anti-HIV-1 gp41 Recombinant Antibody Fab Fragment (4E10-IgG3) | ELISA | Human antibody |
| MRO-026LC-F(E) | Anti-HIV-1 gp41 Recombinant Antibody Fab Fragment (4E10-IgG1) | ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC746 | A*2402/HIV-1 gp41 (RYLKDQQLL) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-4063CQ | Human Anti-HIV-1 gp41 Recombinant Antibody (MRO-4063CQ) | FC | Human IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY129 | Human Anti-HIV-1 gp41 (clone A2) scFv-Fc Chimera | Neut, ADCC | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0625-XY41 | Human Anti-HIV-1 gp41 Vaccinal Antibody (VS-0625-XY41) | ELISA | Human IgG |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: ELISA
-3-1.png)
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, Inhib, FuncS
Application: ELISA, IF, Block, FuncS
-3.jpg)
Application: ELISA, WB
Application: ELISA
Application: ELISA, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
